SrasV1, France, Analysis, bibRecord, 000312

Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA

Identifieur interne : 000312 ( France/Analysis ); précédent : 000311; suivant : 000313

Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA

Auteurs : Serge Cosnier [France] ; Rodica E. Ionescu [France] ; Sebastien Herrmann [France] ; Laurent Bouffier [France] ; Martine Demeunynck [France] ; Robert S. Marks [France]

Source :

Analytical Chemistry [ 0003-2700 ] ; 2006.

RBID : ISTEX:5F3B21B41EEBA49750368605F44AD8BE47734942

Abstract

The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.

Url:

https://api.istex.fr/ark:/67375/TPS-XWTCHZGD-F/fulltext.pdf

DOI: 10.1021/ac060926a

Affiliations:

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ISTEX:5F3B21B41EEBA49750368605F44AD8BE47734942

Le document en format XML

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<front><div type="abstract">The chemical binding of a redox acridone derivative onto a polypyrrole film functionalized by N-hydroxysuccinimide groups provided an electrode capable of anchoring DNA duplex by simple insertion of the grafted acridone intercalator into the dsDNA solution. This electrode was applied for the detection of a ssDNA derived from a West Nile virus sequence. The latter was thus amperometrically detected after its hybridization in solution with a biotinylated complementary oligonucleotide followed by its anchoring and labeling by a glucose oxidase at 1 pg/mL.</div>
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Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA

Electroenzymatic Polypyrrole-intercalator Sensor for the Determination of West Nile Virus cDNA

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Abstract

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